Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy

نویسندگان

چکیده

Fourier lightfield microscopy (FLMic) is a powerful technique to record 3D images of thick dynamic samples. Belonging FLMic the general class computational imaging techniques, its efficiency determined by several factors, like optical system, calibration process, reconstruction algorithm, or computation architecture. In case and algorithm should be fully adapted singular features technique. To this end, concerning reconstruction, we discard use experimental PSFs, propose synthetic one, which calculated on basis paraxial optics taking into account equal influence diffraction pixelation. Using quite simple PSF, performing adequate finally implementing in GPU, demonstrate here possibility obtaining with good results terms resolution strong improvement time. summary, aiming accelerate widespread among users researchers, are proposing fast protocol that very flexible robust against any slight misalignment change element.

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ژورنال

عنوان ژورنال: Optics and Lasers in Engineering

سال: 2023

ISSN: ['1873-0302', '0143-8166']

DOI: https://doi.org/10.1016/j.optlaseng.2022.107336